The quantitative determination of protein and dna is a common analysis contents in biology chemistry and life science . so it is very important to find fluorescence probe with high sensitivity and low detection limit 对蛋白质和dna进行定量测定是生物化学和生命科学中经常涉及的分析内容,建立高灵敏度、低检测限的荧光探针定量测定的方法仍具有重要意义。